Education and training requirements to administer sedation vary by state. The ADA has set forth general sedation guidelines that have been adopted or modified by many states. There are typically dosing limitations involved with anxiolysis , usually a single dose the day of treatment not to exceed the maximum recommended dose (MRD) of the medication in order to achieve the intended level of sedation. However, these sedation laws vary from state to state. Anxiolysis protocols are designed to treat healthy ASA I & II patients ages 18 and up for one to four hours of treatment. Some states now require a permit even for nitrous oxide administration and/or anxiolysis .